Western BLoT Ultra Sensitive HRP Substrate allows detection of very small amounts of protein during Western blot analysis. The Products are manufactured and patented by Cyanagen s.r.l and protected by US7803573 EP1962095 US7855287 EP1950207 CA2742025 EP2405016 and foreign equivalents and other pending patents. It is possible to detect protein levels in the femtogram range using this product.
Western blot chemiluminescence detection series#
#T7104A) is the most sensitive substrate in the series and is suitable for quantitative detection of very low-abundance proteins. The Western BLoT Ultra Sensitive HRP Substrate (Cat.Western BLoT Hyper substrate can also be used for X-ray film detection. Western BLoT Hyper is also appropriate in situations when the amount of primary antibody is limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. #T7103A) is more sensitive than Western BLoT Quant and is suitable for quantitative detection of low-abundance proteins. The Western BLoT Hyper HRP Substrate (Cat.
Western BLoT Quant substrate can also be used for X-ray film detection. In addition, this substrate produces a strong, long-lived signal, allowing long exposure times to enable the detection of low-abundance proteins. The signal from Western BLoT Quant is linear with respect to protein amount over a broad range of concentrations. #T7102A) is intended for Western blot detection using a CCD camera. The Western BLoT Quant HRP Substrate (Cat.Although it is the most basic substrate in Western BLoT HRP substrate series, it produces a strong signal and provides high sensitivity with short exposure times. #T7101A) provides sensitivity suitable for detection using X-ray film. The Western BLoT Chemiluminescence HRP Substrate (Cat.Please refer to the table in the "More Information" section below to compare the substrates' specifications. We offer four types of HRP substrates for Western blot detection. To use, equal volumes of the two solutions are mixed, the blot is incubated with the solution, and chemiluminescence is detected using film or digital imaging equipment. All of the substrates contain two components, a peroxide reagent and a luminol/enhancer solution. The Western BLoT HRP Substrate series are chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting.
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